Table 1. Relative abundance of candidate X-linked genes and known X-linked transgenes in male and female genomic DNA.

Gene	ΔΔCt Male d ± SEM	ΔΔCt Female± SEM	M/F Ratio (95% CI)	p-value M-F/2 = 0 e	p-value M-F = 0 e
Lcaru a	1.0 ± 0.078	2.108 ± 0.129	0.475 (0.354–0.596)	0.616	0.00073
LcATPsynβ a	1.0 ± 0.052	1.006 ± 0.031	0.994 (0.84–1.148)	0.00026	0.925
LcCG1970 a	1.0 ± 0.043	2.179 ± 0.128	0.459 (0.373-.0545)	0.298	0.00033
LcEph b	1.0 ± 0.048	1.986 ± 0.092	0.503 (0.417–0.589)	0.92	0.00022
LcEphrin b	1.0 ± 0.028	1.076 ± 0.038	0.930 (0.822–1.037)	0.00004	0.168
Lcgw a	1.0 ± 0.047	2.284 ± 0.111	0.438 (0.362–0.514)	0.108	0.00013
Lchsp83 c	1.0 ± 0.017	0.953 ± 0.035	1.049 (0.94–1.158)	0.00001	0.281
LcJwa	1.0 ± 0.0549	2.08 ± 0.0506	0.481 (0.406–0.555)	0.5368	0.00003
LcSlp1 b	1.0 ± 0.066	2.127 ± 0.042	0.470 (0.386–0.553)	0.401	0.00003
LcThd1 b	1.0 ± 0.128	2.088 ± 0.130	0.479 (0.304–0.654)	0.771	0.0019
Lczfh2 b	1.0 ± 0.071	2.030 ± 0.053	0.493 (0.397–0.589)	0.85	0.00008
RFPex b	1.0 ± 0.062	1.775 ± 0.036	0.563 (0.468–0.657)	0.142	0.00012
ZsGreen c	1.0 ± 0.039	1.938 ± 0.111	0.516 (0.424–0.608)	0.667	0.0005

^a,b,cSuperscript indicates strain that was the source of genomic DNA for analysis.

^a: wild type,

^b: SLAM5, and

^c: DR3-9.

^d. For each gene the male sample was chosen as the control/ calibrator sample and set to equal 1.0.

^e. Two p-values for each gene were calculated from the competing hypotheses that either the Male-Female/2 difference was zero or that the Male-female difference was zero. The latter would be expected for a M/F ratio of one.