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. 2015 Oct 27;10(10):e0141544. doi: 10.1371/journal.pone.0141544

Table 1. Relative abundance of candidate X-linked genes and known X-linked transgenes in male and female genomic DNA.

Gene ΔΔCt Male d ± SEM ΔΔCt Female± SEM M/F Ratio (95% CI) p-value M-F/2 = 0 e p-value M-F = 0 e
Lcaru a 1.0 ± 0.078 2.108 ± 0.129 0.475 (0.354–0.596) 0.616 0.00073
LcATPsynβ a 1.0 ± 0.052 1.006 ± 0.031 0.994 (0.84–1.148) 0.00026 0.925
LcCG1970 a 1.0 ± 0.043 2.179 ± 0.128 0.459 (0.373-.0545) 0.298 0.00033
LcEph b 1.0 ± 0.048 1.986 ± 0.092 0.503 (0.417–0.589) 0.92 0.00022
LcEphrin b 1.0 ± 0.028 1.076 ± 0.038 0.930 (0.822–1.037) 0.00004 0.168
Lcgw a 1.0 ± 0.047 2.284 ± 0.111 0.438 (0.362–0.514) 0.108 0.00013
Lchsp83 c 1.0 ± 0.017 0.953 ± 0.035 1.049 (0.94–1.158) 0.00001 0.281
LcJwa 1.0 ± 0.0549 2.08 ± 0.0506 0.481 (0.406–0.555) 0.5368 0.00003
LcSlp1 b 1.0 ± 0.066 2.127 ± 0.042 0.470 (0.386–0.553) 0.401 0.00003
LcThd1 b 1.0 ± 0.128 2.088 ± 0.130 0.479 (0.304–0.654) 0.771 0.0019
Lczfh2 b 1.0 ± 0.071 2.030 ± 0.053 0.493 (0.397–0.589) 0.85 0.00008
RFPex b 1.0 ± 0.062 1.775 ± 0.036 0.563 (0.468–0.657) 0.142 0.00012
ZsGreen c 1.0 ± 0.039 1.938 ± 0.111 0.516 (0.424–0.608) 0.667 0.0005

a,b,cSuperscript indicates strain that was the source of genomic DNA for analysis.

a: wild type,

b: SLAM5, and

c: DR3-9.

d. For each gene the male sample was chosen as the control/ calibrator sample and set to equal 1.0.

e. Two p-values for each gene were calculated from the competing hypotheses that either the Male-Female/2 difference was zero or that the Male-female difference was zero. The latter would be expected for a M/F ratio of one.