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. 2015 Oct 27;10(10):e0141365. doi: 10.1371/journal.pone.0141365

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© 2015 Moharil et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A, C, E, G, I, K, M, O) hBM-MSCs or (B, D, F, H, J, L, N, P) hHF-MSCs were transduced with the LV library of 27 Pr/RE and coaxed to differentiate into SMCs. The activities of the (A, B) SMAD2/3-RE; (C, D) SMAD7-RE; (E, F) CArG-RE; (G, H) CArGA-RE; (I, J) KLF4-RE; (K, L) ACTA2-Pr; (M, N) SM22-Pr; and (O, P) rMYH11-Pr were determined by fluorescence microscopy and the normalized intensity was plotted as a function of time. MSCs cultured in GM served as a control. * indicates p < 0.05 between DM and GM as determined by Student’s two-tailed t-test at individual time points. + indicates statistical significance of the Pr/RE activities under DM vs GM evaluated over entire curve by growth curve analysis (p < 0.05).