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. 2015 Oct 27;10(10):e0141365. doi: 10.1371/journal.pone.0141365

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© 2015 Moharil et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — Dynamics of Pr/RE activity in hHF-MSCs cultured in DM containing chemical inhibitors. (A, B) 10 μM SB431542 (SB4); (C, D) 10 μM CCG1423 (CCG); (E, F) 10 μM Y27632 (Y27); (G, H) 20 μM SB203580 (SB2); (I, J) 10 μM SP600125 (SP6). MSCs cultured in GM served as negative control and MSCs cultured in DM served as positive control. Normalized activities of (A, C, E, G, I) CArG-RE, and (B, D, F, H, J) ACTA2-Pr are shown. The normalized values were scaled from 0–1 and plotted as a function of time. * indicates p < 0.05 between DM and GM as determined by Student’s two-tailed t-test at individual time points. + indicates statistical significance of the Pr/RE activities under DM vs GM evaluated over the entire curve by growth curve analysis (p < 0.05).