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. 2015 Oct 29;9:385. doi: 10.3389/fnins.2015.00385

Figure 5.

Figure 5

Electron micrographs of the localization of biotin ethylenediamine (BED) in blood vessels deep inside the cortex of a 2-month-old opossum 10 min after an intravenous injection. Similar staining is found after an intravenous injection of biotin-dextran (BDA3000). (A) Low-power micrograph showing two paired vessels with abundant reaction product within lumen. No reaction product is visible in the surrounding tissue. Pairs of arteries and veins are characteristic of the vascular pattern in marsupial brains (Wislocki and Campbell, 1937). (B) High-power micrograph of an interendothelial cleft showing that the tight junctions in the young adult restrict the passage of BED through the cleft (arrowhead). Scale bar = 4 μm in (A); 300 nm in (B). From Ek et al. (2006).