Figure 6.
Cellular localization of dextran probes in postnatal lateral ventricular choroid plexus of the marsupial South American opossum (Monodelphis domestica). (A) Forty-five minutes after intraperitoneal injection with BDA-3 kDa, the probe can be seen in individual epithelial cells of the choroid plexus (filled arrow), as well as in the blood vessel lumen (arrowhead) and precipitated in the CSF (unfilled arrow). (B) Ten minutes after intraventricular injection with BDA-3 kDa–Fluorescein, more epithelial cells take up the probe (filled arrows) following CSF injection compared with intraperitoneal injection (A). Penetration of the fluorescent probe between epithelial cells is stopped by the presence of tight junctions (examples highlighted by arrowheads). Scale: 50 μm. From Liddelow et al. (2009).