Table 1.

Effect of albumin (Alb) molar concentration on binding of Evans blue dye (T-1824).

Albumin Molar conc.	T-1824 Molar conc.	Unbound T-1824 Molar conc.	T-1824:Alb Molar ratio	Unbound, T-1824(%)
1.23E-04	3.50E-03	2.13E-03	28.5	60.9
3.77E-04	1.76E-03	5.42E-06	4.7	0.31
5.03E-04	8.80E-04	1.40E-06	1.7	0.16
5.77E-04	3.52E-04	4.37E-07	0.61	0.12
6.04E-04	1.76E-04	2.00E-07	0.29	0.11
6.27E-04	1.76E-05	1.91E-08	0.03	0.11

Data in the first three columns are from the study of Allen and Orahovats (1950) in which 4 ml of a 0.43% solution of T-1824 was mixed with different volumes of a 4.4% albumin solution. At a ratio of 28.5 moles of T-1824 per mole of albumin, only around 40% of the T-1824 is actually bound to albumin (i.e., each albumin molecule binds a maximum of around 10 molecules of T-1824). Note that at all molar ratios less than this maximum binding capacity, a small proportion of T-1824 (0.11–0.31%) remains free in solution unbound to albumin.