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. 2015 Oct 28;10(10):e0141669. doi: 10.1371/journal.pone.0141669

Fig 3. Reversal of inhibitory effects of PD-L1, IDO and TGF-β by pharmacological means.

Fig 3

(A) Analysis of CD3+ T cell proliferation after 120 h of co-culture with control EpCAM+ CHO cells and IDO-expressing, EpCAM+ CHO cells in the presence or absence of 500 μM tryptophane (Trp) with and without 1 μg/ml AMG 110. (B) Dose-dependent, redirected target cell lysis of parental EpCAM+ CHO cells and EpCAM+ CHO TGF-β transfectants +/- 50 μg/ml TGF-β neutralizing anti-human TGF-β antibody in presence of AMG 110 and CD3+ T cells in an E:T ratio of 4:1 after 72 h incubation. (C) Dose-dependent redirected target cell lysis of control EpCAM+ CHO cells and EpCAM+ CHO PD-L1 transfectants with and without 5 μg/ml of an anti-human PD-L1-neutralizing antibody in the presence of AMG 110 and pre-stimulated CD8+ T cells. The E:T ratio was 1:1, the assay duration 24 h.