Fig 4. Combinatorial effect of evasion mechanisms on AMG 110-mediated redirected target cell lysis.

Parental human EpCAM⁺ CHO cells and different combinations of EpCAM⁺ CHO cells expressing evasion proteins were co-cultured at an E:T ratio of 4:1 with CD3⁺ T cells and various AMG 110 concentrations for 72 h. For each curve an equal amount of control EpCAM⁺ CHO cells was replaced by an additional stable CHO cell line until the entire target cell population comprised evasion protein-expressing cells and contained 1 mM adenosine. (A) Target cell lysis was determined by a FACS-based cytotoxicity assays. Sigmoidal dose-response curves were created with GraphPad Prism software. Error bars represent SEM values. (B) Relative change in EC₅₀ and (C) percentage of cell lysis after 72 h were calculated as described in Fig 3. Error bars represent SEM values from two independent experiments