Table 6.
Multiplexing | Methods | Targets | References |
---|---|---|---|
Simplex | Element-specific | p35S and CP4-EPSPS | [65] |
Triplex | Taxon-specific | Zein | [66] |
Event-specific | MIR604 and MON88017 | ||
Tetraplex | Event-specific | Bt176, MON810, NK603, and GA21 | [66] |
Tetraplex | Event-specific | Bt11, T25, MIR162, and MON89034 | [66] |
Pentaplex | Taxon-specific | SPS | [67] |
Element-specific | Cry1Ac, tNOS, p35S, and LS28 | ||
Hexaplex | Taxon-specific | SAD1 | |
Element-specific | Cry1Ac, Cry1F, and pat | [68] | |
Event-specific | 281-24-236 and 3006-210-23 |
CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain); Cry (gene encoding the Bacillus thuringiensis δ-endotoxin); LS28 (choline kinase); p35S (promoter of the 35 S cauliflower mosaic virus) SAD1 (stearoyl-acyl carrier protein desaturase gene from cotton); SPS (sucrose phosphate synthase gene from rice); tNOS (terminator of the nopaline synthase gene); tORF23 (open reading frame 23 terminator from A. tumefaciens); Zein (Zein gene from maize).