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Immunohistochemistry Used to Display Fate Commitment by Generation on the Lineage Tree
Stain images for β-Tubulin (red, neuron) (A) and Nestin (cyan, neural progenitor cell, NPC, B). The final frame of live-cell time-lapse sequence (C) with segmentation and tracking overlaid and stain results blended. The cell fate commitment is shown on the lineage tree colored by the generation (in parenthesis) when all offspring take on the same fate.
