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. 2015 Oct 15;2015:865053. doi: 10.1155/2015/865053

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Copyright © 2015 Yoshihiko Inaoka et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Two intra-assays of intraindividual assay in the human liver tissues (a) and intracellular assay in three culture cell lines (b and c). (a) Relative amount of AHSG ^∗ 1 mRNA to that of AHSG ^∗ 2 was obtained in genomic DNA (left) and mRNA from the liver tissue (right). Bars represent the mean ± SD of four specimens. (b) Nondenaturing polyacrylamide gel electrophoresis of allele-specific RT-PCR for ^∗ 1, ^∗ 2, and β-actin products. Lane 1: HeLa; lane 2: HepG2; and lane 3: PLC/PRF/5. (c) Comparison of allelic mRNA amount of AHSG ^∗ 1 and AHSG ^∗ 2 to β-actin. N.D. denotes not detected. Bars represent the mean ± SD in triplicate; ^※ P < 0.01 significant versus the amount of AHSG ^∗ 1.