Figure 1.

Generation and Characterization of iPSCs from WT and oc/oc Mice

(A) Experimental outline for the generation of WT and oc/oc iPSC clones with excised reprogramming vector (VCN = 0) and carrying the correct chromosome complement (see text for details).

(B) Expression of alkaline phosphatase on WT and oc/oc iPSC colonies.

(C) Expression of Oct4, Sox2, Nanog, and SSEA-1 on WT and oc/oc iPSCs as revealed by immunofluorescence. Nuclei are stained with DAPI. Scale bars, 100 μm.

(D) Karyotype analysis of WT and oc/oc iPSC clones.

(E) Generation of the three germ layers in vitro by differentiated WT and oc/oc iPSCs is revealed by immunofluorescence. Expression of Brachyury, AFP, and Nestin indicate formation of mesoderm, endoderm, and ectoderm, respectively. Scale bars, 50 μm.

(F) H&E staining of teratomas generated after subcutaneous injection of WT and oc/oc iPSC clones into NSG mice, demonstrating differentiation into the three germ layer derivatives (i, mesoderm-cartilage; ii, mesoderm-adipose tissue; iii, endoderm-glandular structure; iv, endoderm-glandular structures (intestinal paneth or pancreatic acinar cell-like); v, ectoderm-primitive and mature neuroepithelium; vi, ectoderm-primitive neuroepithelium). Scale bars, 50 μm.

See also Figure S1.