Figure 4.

Differentiated iPSCs Generate Functional Osteoclasts

(A) Images of TRAP⁺ osteoclasts derived from iPSCs (i, iv, and vi) and from fresh mouse tissues (ii and v, spleen; iii, BM). Osteoclasts are defined as giant, multinucleated TRAP⁺ cells. Results are representative of at least three independent experiments/group. Scale bars, 100 μm.

(B) Representative images of dentine discs stained with toluidine blue, showing the presence of resorption pits in dentine discs previously seeded with osteoclast precursors obtained from WT or BAC-corrected oc/oc iPSCs (i and iv, respectively) and from the BM of a WT mouse (ii), but not in discs seeded with the non-corrected oc/oc 62 iPSC clone (iii). Results are representative of at least three independent experiments/group. Scale bars, 100 μm.