Figure 1.

Identification and Prospective Isolation of HuSCs

(A) Cell-sorting scheme used to isolate huSCs by FACS for a representative specimen of latissimus dorsi muscle. Red gates indicate subpopulations containing huSCs. Numbers indicate percentage of total events falling within each gate; given error represents SD (n = 15).

(B) Immunofluorescence (IF) analysis of PAX7 expression in purified huSCs at low (top) and high (bottom) magnification 3 days after isolation. Cells were stained with antibodies against PAX7 and with DAPI to identify nuclei.

(C) qRT-PCR analysis of PAX7, PAX3, MYF5, MYOD, MYOG, and MEF2C mRNA levels in the huSC and huSC-depleted cell populations after a period of 7 days in culture is shown (n = 4). Error bars represent SD. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.