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. 2015 Sep 10;5(4):569–581. doi: 10.1016/j.stemcr.2015.08.007

Figure 1.

Figure 1

Human iBAs Induced from iPSCs

(A and B) Human iPSCs were cultured in the presence of RA followed by transduction with PRDM16 retroviral vector. After culturing for 12 days, cells were subjected to Oil Red O and mitochondrial staining (A), as well as immunostaining with the indicated antibodies (B). Original magnification was 100×.

(C) Real-time RT-PCR was performed to evaluate mRNA levels of the indicated genes in HDFs and hiBAs.

(D) Human dermal fibroblasts (HDFs) and human iBAs were stimulated with the indicated concentrations of isoproterenol. Twelve hours later, UCP1 mRNA levels were evaluated by real-time RT-PCR.

(E) Oxygen consumption of the indicated cells was evaluated. Oligomycin (O), FCCP (F), and antimycin A and rotenone (A + R) were serially added to the culture at the indicated time periods.

In (A)–(E), n = 3 cultures per group. In (C) and (D), values (average ± SD) were normalized to β-ACTIN mRNA and expressed relative to values for the HDFs (set to 1.0; n = 3 cultures per group). In (E), values are average ± SD (n = 3 cultures per group). The experiments were repeated more than three times.