Figure 2.

iBAs Suppressed Diet-Induced Obesity and Diabetes in Mice

(A and B) C57BL/6 mice were subcutaneously transplanted with syngenic iBAs or control cells that had not been transduced with Prdm16 (d0). Two weeks after the transfection, thermographic images of mice were captured after exposure to 4°C for 2 hr (A). Mice were then sacrificed, and graft tissue sections were stained with Oil Red O (B; original magnification was 40×).

(C and D) C57BL/6 mice were subcutaneously transplanted with syngenic iBAs or GFP-transduced cells as control and housed on either standard- or high-calorie diet. Body weights of mice were measured (C), and serum lipid concentrations were determined 4 weeks after transplantation (D).

(E–H) KK-Ay diabetic mice were subcutaneously transplanted with syngenic iBAs or GFP-transduced fibroblasts as a control. Body weights (E) and non-fasting serum glucose levels (F) of the mice are shown. Urine glucose levels were measured 3 weeks after the transplantation (G), and serum lipid concentrations were determined 4 weeks after the transplantation (H).

In (C)–(H), values are average ± SD (n = 8 mice per group) for one out of two independent experiments. ^∗p < 0.05 (two-sided Student’s t test). In (A) and (B), n = 3 mice per group. The experiments were repeated more than twice. See also Figures S1 and S2.