Table 1. Description of candidate reference genes from Lilium davidii var. unicolor for qRT-PCR analysis.

Internal gene	Gene name	Accession number	Primer sequence (5’-3’) forward/reverse	Amplicon length (bp)	Amplification efficincy (%)	Regression coefficient (R2)
α-TUB	Alpha-tubulin	KP861877	TGGCTTCACAGTCTATCCCTC/ GGGACAAGATTGGTCTGGAAC	282	98.96	0.9937
β-TUB	Beta-tubulin	KP861875	CTATGACATCTGTTTCCGCACTC/ AGCGATACTGTTGGGAGCCT	227	96.30	0.9990
ACT	Actin	KP861871	ATCTATGAGGGTTATGCTCTTCC/ CATCAGGCAGCTCGTAACTTC	241	100.91	0.9948
AP4	AP-4 complex subunit	KP861878	GATGGGGCTTCTTTATACGGT/ TCATTACAGCAAACTCTCCCTCT	163	104.31	0.9946
eIF	Eukaryotic initiation factor 1α	KP861874	TATGGTGAGCTTCCTGACAACGT/ TCACAAAGACAGTAACAACAGCGAT	265	97.09	0.9998
FP	F-box family protein	KP861876	TCGCCTACATCGCTAACC/ TTCCCAATAATCGCAAGACC	169	99.23	0.9961
GAPDH	Glyceraldehyde-3- phosphate dehydrogenase	gb|KP179417.1|	GCTGCAAGTTTCAACATTATTCC/ ATCCTCATCAGTATAACCAAGA	240	100.50	0.9910
RH2	DEAD box RNA helicase	KP861880	CCGAGACCAGTTCGTTCA/ ACAATAGGACCATCCCCAT	242	99.82	0.9994
UBQ	Ubiquitin	KP861873	TATGGTGGATTATCGGTTTCTACTG/ ACCACAGACTTTTTCAGTATCGCA	293	99.61	0.9959
UBC	Ubiquitin-conjugating enzyme	KP861872	GAGTGGAGCGTGACCATAAT/ CTGGTGGATGCAGAATTGAT	184	98.99	0.9985
18S	18S ribosomal RNA	gb|AY684927.1|	CGTTTCGGGCATGATTTGTGG/ TCGCATTTCGCTACGTTCTTC	183	96.82	0.9963
60S	60S ribosomal RNA	KP861879	GCAAAGGCTGTCAAAAATCAGGTAG/ ATAACCCACAAACTAATAGCCCTGC	156	98.39	0.9916