Table 3. Ranking of reference genes and their expression stability values calculated using BestKeeper.
Rank | Total (A) | Organs (B) | Leaves in developmental process (C) | Scales in developmental process (D) | Leaves under stress (E) | Scales under stress (F) | Roots under stress (G) | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene name | Stability value | Gene name | Stability value | Gene name | Stability value | Gene name | Stability value | Gene name | Stability value | Gene name | Stability value | Gene name | Stability value | |
1 | FP | 0.424 | GAPDH | 0.298 | RH2 | 0.173 | eIF | 0.218 | FP | 0.212 | FP | 0.169 | UBC | 0.368 |
2 | AP4 | 0.634 | ACT | 0.325 | ACT | 0.208 | β-TUB | 0.272 | UBC | 0.428 | ACT | 0.298 | AP4 | 0.556 |
3 | RH2 | 0.682 | 60S | 0.334 | α-TUB | 0.291 | FP | 0.300 | UBQ | 0.489 | GAPDH | 0.555 | FP | 0.626 |
4 | UBC | 0.696 | UBC | 0.338 | UBQ | 0.447 | AP4 | 0.318 | AP4 | 0.580 | RH2 | 0.734 | GAPDH | 0.632 |
5 | UBQ | 0.703 | UBQ | 0.382 | FP | 0.528 | 60S | 0.319 | ACT | 0.618 | AP4 | 0.781 | ACT | 0.696 |
6 | GAPDH | 0.708 | FP | 0.430 | β-TUB | 0.576 | GAPDH | 0.347 | 60S | 0.629 | β-TUB | 0.943 | RH2 | 0.720 |
7 | β-TUB | 0.842 | AP4 | 0.461 | AP4 | 0.630 | ACT | 0.399 | eIF | 0.702 | eIF | 1.002 | α-TUB | 0.822 |
8 | ACT | 0.874 | RH2 | 0.551 | GAPDH | 0.745 | UBC | 0.484 | GAPDH | 0.824 | UBC | 1.029 | UBQ | 0.831 |
9 | eIF | 0.972 | β-TUB | 0.578 | 60S | 0.823 | UBQ | 0.516 | RH2 | 0.844 | α-TUB | 1.122 | β-TUB | 0.906 |
10 | α-TUB | 1.074 | eIF | 0.855 | UBC | 0.859 | RH2 | 0.668 | α-TUB | 0.862 | UBQ | 1.210 | eIF | 1.039 |
11 | 60S | 1.100 | α-TUB | 0.964 | eIF | 1.235 | α-TUB | 1.258 | β-TUB | 0.923 | 60S | 1.289 | 60S | 1.126 |
12 | 18S | 2.024 | 18S | 2.019 | 18S | 1.881 | 18S | 3.52 | 18S | 2.738 | 18S | 1.340 | 18S | 1.604 |