Skip to main content
. 2015 Oct 28;10(10):e0141323. doi: 10.1371/journal.pone.0141323

Table 3. Ranking of reference genes and their expression stability values calculated using BestKeeper.

Rank Total (A) Organs (B) Leaves in developmental process (C) Scales in developmental process (D) Leaves under stress (E) Scales under stress (F) Roots under stress (G)
Gene name Stability value Gene name Stability value Gene name Stability value Gene name Stability value Gene name Stability value Gene name Stability value Gene name Stability value
1 FP 0.424 GAPDH 0.298 RH2 0.173 eIF 0.218 FP 0.212 FP 0.169 UBC 0.368
2 AP4 0.634 ACT 0.325 ACT 0.208 β-TUB 0.272 UBC 0.428 ACT 0.298 AP4 0.556
3 RH2 0.682 60S 0.334 α-TUB 0.291 FP 0.300 UBQ 0.489 GAPDH 0.555 FP 0.626
4 UBC 0.696 UBC 0.338 UBQ 0.447 AP4 0.318 AP4 0.580 RH2 0.734 GAPDH 0.632
5 UBQ 0.703 UBQ 0.382 FP 0.528 60S 0.319 ACT 0.618 AP4 0.781 ACT 0.696
6 GAPDH 0.708 FP 0.430 β-TUB 0.576 GAPDH 0.347 60S 0.629 β-TUB 0.943 RH2 0.720
7 β-TUB 0.842 AP4 0.461 AP4 0.630 ACT 0.399 eIF 0.702 eIF 1.002 α-TUB 0.822
8 ACT 0.874 RH2 0.551 GAPDH 0.745 UBC 0.484 GAPDH 0.824 UBC 1.029 UBQ 0.831
9 eIF 0.972 β-TUB 0.578 60S 0.823 UBQ 0.516 RH2 0.844 α-TUB 1.122 β-TUB 0.906
10 α-TUB 1.074 eIF 0.855 UBC 0.859 RH2 0.668 α-TUB 0.862 UBQ 1.210 eIF 1.039
11 60S 1.100 α-TUB 0.964 eIF 1.235 α-TUB 1.258 β-TUB 0.923 60S 1.289 60S 1.126
12 18S 2.024 18S 2.019 18S 1.881 18S 3.52 18S 2.738 18S 1.340 18S 1.604