Figure 4.

Differentiation of Cholangiocytes from hiPSC-Derived CPM⁺ LPCs

(A) Schematic image of cholangiocyte-like cells differentiation from CPM⁺ LPCs.

(B) Phase contrast image. CPM⁺ LPCs form cysts in collagen/Matrigel after 7 days of culture. See also Figures S4A and S4B.

(C) Expression of AFP in CPM⁺ LPCs and CPM⁺ cholangiocytes. The results are shown as the mean ± SEM of four independent experiments (each experiment contains two technical replicates). ^∗∗p < 0.01.

(D–F) Immunofluorescence staining for cholangiocyte markers in CPM⁺ cholangiocyte-like cells. Localization of (D) CK7 (red), F-actin (green), (E) CD49f (red), PKC (green), (F) β-catenin (red), AQP1 (green). Nuclei were visualized by Hoechst 33342 staining (blue). Scale bars, 100 μm.

(G) Gene expression profile of CPM⁺ cholangiocyte-like cells compared with iPSC cholangiocyte-like cells. The results are shown as the mean ± SEM of four independent experiments (each experiment contains two technical replicates). ND, not detected. ^∗∗p < 0.01, ^∗∗∗p < 0.001.

See also Tables S1 and S2.