Figure 2.

Key Steps of the Human iPSC Manufacturing Process

The manufacturing of human iPSCs under defined and cGMP conditions include tissue acquisition to obtain a fresh cord blood unit, CD34+ cells isolation from the cord blood, reprogramming of CD34+ cells into iPSCs using the 4D nucleofector system and episomal-based technology, and expansion and banking of iPSCs. The photomicrographs represent CD34+ cells isolated from cord blood and expanded in culture (CD34+ Isolation and Priming), iPSC colonies on day 10 post-nucleofection (P0 Colonies), and iPSCs at passage 6 (P6 Colonies) passage 12 (P12 Culture). Scale bars, 500 μm, except in the CD34+ Isolation and Priming image (250 μm).