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. 2015 Sep 24;5(4):490–498. doi: 10.1016/j.stemcr.2015.08.014

Figure 1.

Figure 1

Efficient Differentiation of hES Cells into DE

(A) Scheme of endodermal differentiation.

(B) Flow cytometry analysis of hES cells and D0, D1, and D3 of endoderm differentiation cultures with anti-SOX17 and anti-FOXA1 antibodies.

(C) (Left) Venn diagram of OCT4 and bivalent domain occupancy in hES cells. (Right) GO terms from the gene set overlapping OCT4 and bivalent domains.

(D) Anti-OCT4.

(E) Anti-EZH2.

(F) Anti-SUZ12.

(G) Anti-H3K27me3 ChIP on H9 D0 and D1 cells. Sites used for ChIP-qPCR are indicated in kilobases (K) from the transcription start site. The IgG control for each ChIP is also shown. Data shown are an average from three independent experiments. Statistical significance indicated as ∗p < 0.05. Activin A, Act; input, INP.