Table 3. CT values of 4 mixtures of M. pneumoniae type 1 and type 2 DNA analyzed by duplex real-time PCR.

	CT values by mixed DNA analysis		CT values by individual DNA analysis
Composition of M. pneumoniae DNA mixture a	ATCC 29342 (Type 1)	ATCC 15531 (Type 2)	ATCC 29342 (Type 1)	ATCC 15531 (Type 2)
A	26.09	N b	24.60	36.66
	25.89	N	24.72	35.37
	25.74	N	24.41	35.77
B	N	23.43	38.26	22.69
	N	23.45	37.83	22.97
	N	22.99	38.42	22.82
C	26.04	23.53	24.60	22.69
	25.89	23.37	24.72	22.97
	25.78	23.12	24.41	22.82
D	N	41.12	38.26	36.66
	39.77	38.86	37.83	35.37
	N	N	38.42	35.77

^a A: 8 pg ATCC 29342+9 fg ATCC 15531; B: 8 fg ATCC 29342+9 pg ATCC 15531; C: 8pg ATCC 29342+9 pg ATCC 15531; D: 8 fg ATCC 29342+9 fg ATCC 15531.

^b N, negative result.