Fig 1. Characterization of circulating CD14⁺CD169⁺ monocytes in CRC patients.

Peripheral blood mononuclear cells (PBMCs) were obtained from individual subjects and stained with anti-CD14 and anti-CD169. The frequency of circulating CD14⁺CD169⁺ monocytes was characterized by flow cytometry. The cells were gated initially on mononuclear cells and then on CD14⁺ cells. Subsequently, the percentages of CD14⁺CD169⁺ monocytes were determined. To characterize different subsets of CD14⁺CD169⁺ monocytes, PBMCs were stained with fluorescent antibodies against CD14, CD169 and CD163, CD206 or MAC387. The cells were gated on CD14⁺CD169⁺ monocytes and the percentages of CD14⁺CD169⁺CD163⁺, CD14⁺CD169⁺CD206⁺ and CD14⁺CD169⁺MAC387⁺ macrophages were determined. Data are representative charts of flow cytometry and expressed as the values of individual subjects. The horizontal lines indicate the median for individual groups. (A) Flow cytometry analysis of CD14⁺CD169⁺ monocytes/macrophages. (B) Flow cytometry analysis of different subsets of circulating CD14⁺CD169⁺ monocytes/macrophages. (C) The percentages of CD14⁺CD169⁺ monocytes. (D) The frequency of CD14⁺CD169⁺CD163⁺ macrophages. (E) The frequency of CD14⁺CD169⁺CD206⁺ macrophages. (F) The frequency of CD14⁺CD169⁺MAC387⁺ macrophages.