Fig 4. Demethylation of HPV16 LCR CpG sites and knockdown of E6 and E7 inhibit cell growth in HPV-positive cancer cells.

A) After demethylation of the CpG sites, cell growth was strongly inhibited in UM-SCC47 and CaSki cells, but not in SiHa cells. B) E6 and E7 expression was significantly decreased after siRNA transfection. C) Cell growth was inhibited at 96 h after E6 and E7 knockdown. All data are means ± SEM from 3 independent experiments in A) and C), and means ± SD from 3 independent experiments in B).