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. 2015 Oct 29;6:933. doi: 10.3389/fpls.2015.00933

FIGURE 1.

FIGURE 1

Transient expression of AvrLm1 triggers LepR3-mediated cell death in Nicotiana benthamiana leaves. (A) N. benthamiana leaves Agro-infiltrated with the LepR3, PR1a-AvrLm1, and GFP constructs in the order indicated on the image. To ensure secretion of AvrLm1 to the apoplastic space, the indigenous AvrLm1 signal peptide was replaced by the tobacco PR1a signal peptide and the construct was designated as PR1a-AvrLm1. Truncated AvrLm1 lacking its indigenous signal peptide (ΔspAvrLm1) was also included. Co-expression of LepR3 or PR1a-AvrLm1 with GFP served as negative controls. The experiment was performed three times and each time 10 plants were infiltrated. (B) N. benthamiana leaves Agro-infiltrated with PR1a-AvrLm1/LepR3, AvrLm1/LepR3, AvrLm1/GFP, and LepR3/GFP constructs in the order indicated in the image. The numbers under the panels indicate the total occurrence of HR within 30 sites co-infiltrated with PR1a-AvrLm1/LepR3. Images are from photographs taken 6 days after infiltration.