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. 2015 Apr 7;22(6):752–759. doi: 10.1016/j.sjbs.2015.04.004

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Necrosis like effect of Acanthopanax sessiliflorus stem bark extracts on human breast cancer cells. Following 24 h treatment with ASSBE-nHF, both floating and adherent cells were harvested, stained with annexin V-FITC and propidium iodide and then the percentage of necrotic and apoptotic cells in each group was analysed by flow cytometry. The representative fluorescent histogram showing percentage distribution of viable (Q1), early apoptotic (Q4), late apoptotic (Q3) and dead (Q2) cells in control and ASSBE-nHF treated groups.