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Effect of Acanthopanax sessiliflorus stem bark extracts on the nuclear morphology of human breast cancer cells. Cells were treated with ASSBE-nHF for 12 h. To assess the nuclear morphology, cells were fixed with 4% formaldehyde and stained with Hoechst 33342, and then the images were captured using fluorescence microscopy (400× magnification). The arrows red point to the necrotic nucleus.
