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. 2015 Apr 7;22(6):752–759. doi: 10.1016/j.sjbs.2015.04.004

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Effect of Acanthopanax sessiliflorus stem bark extracts on the levels of apoptosis markers in human breast cancer cells. Representative western blotting data show the effect of ASSBE-nHF on the levels of caspases and GAPDH proteins in MCF-7 and MDA-MB-231 cells. The cells were treated with ASSBE-nHF (100 μg/ml) for 0, 12 and 24 h, and then immunoblotting for caspases was performed using specific antibodies (Novusbio and Cell Signalling Technology) in automated WES Proteinsimple instrument with GAPDH (Santa cruz, sc-25778) as a loading control.