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. 2015 Oct 29;5:15898. doi: 10.1038/srep15898

Table 3. Primers used for PCR and qPCR.

Organism Target gene Primer name Primer sequences (5′ → 3′) Length (bp) Reference
PCR
 Portiera 16S rRNA Por-F Por-R GGAAACGTACGCTAATAC TGACGACAGCCATGCAGCAC ~900 77
 Hamiltonella 16S rRNA Ham-F Ham-R TGAGTAAAGTCTGGGAATCTGG AGTTCAAGACCGCAACCTC ~750 78
qPCR
 Portiera 16S rRNA Port73-F Port266-R GTGGGGAATAACGTACGG CTCAGTCCCAGTGTGGCTG ~200 79
 Hamiltonella 16S rRNA H-16S-Fis H-16S-Ris GCATCGAGTGAGCACAGTTT TATCCTCTCAGACCCGCTAGA ~240 39
 B. tabaci β-actin Actin-F Actin-R TCTTCCAGCCATCCTTCTTG CGGTGATTTCCTTCTGCATT ~200 80