Figure 10. Phenylalanine-induced dendritic sprouting alterations in hippocampal neurons prevented by doxycycline co-treatment.

Hippocampal “sandwich” co-cultures were treated with Phe/Doxy for 72 h. The neuron fractions were stained with NF200 and nuclei were marked by Hoechst 33258. At least eight fields (600x) for each condition from three independent experiments were analysed. The number of dendritic branches (A) and Sholl intersections (B) was significantly decreased by Phe treatments. Treatment with 25 μM Doxy significantly counteracted the dendritic alterations induced by 20 mM Phe. *p < 0.05, **p < 0.01, ***p < 0.001 versus control. °p < 0.05 vs 20 mM Phe. One-way ANOVA and Tukey’s post-test.