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. 2015 Oct 29;5:15603. doi: 10.1038/srep15603

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Copyright © 2015, Macmillan Publishers Limited

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(a) The −/− pigs have a smaller body size. (b) Western blot of GHR in F2 pigs. The −/−, +/− (GHR^+/4bp) and +/+ (GHR^+/+) represent different genotypes in the F2 generation. The expected sizes for pig GHR are 100 kDa (glycosylated) or 70 kDa (un-glycosylated); GAPDH was used as an internal control. The mRNA expression of GHR (c), IGF-I (d), JAK2 (e) and STAT5b (f) genes in the F2 generation was determined by qRT-PCR. The expression levels were determined by normalizing the expression relative to GAPDH. The data were combined from three independent experiments; the bars represent the means ± SD (at least three pigs per group); ***P < 0.001, *P < 0.05.