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. 2015 Oct 29;5:15575. doi: 10.1038/srep15575

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Copyright © 2015, Macmillan Publishers Limited

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MDBK cells were transfected with either non-targeting control (NC) siRNA or siRNA targeting HO-1, for 12 h followed by infection with BVDV at a MOI of 0.1 for 36 h. Reduced HO-1 expression was verified by Western blot analyze using antibody against HO-1 (A). Virus titers (B) and BVDV RNA (C) in the supernatants were analyzed by CCID₅₀ and qRT-PCR. Data are expressed as mean ± s.d. of three independent experiments, **P < 0.01, and ***P < 0.001.