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. 2015 Oct 29;5:15688. doi: 10.1038/srep15688

Figure 1. Grb2 is a TGF-β2-target gene in Theileria-infected macrophages.

Figure 1

(A) Total RNA was extracted from virulent (V) and attenuated (A) macrophages. Relative mRNA levels of the different genes were determined by qPCR and normalized to GAPDH transcripts. Left panel. The transcription level of Grb2 is higher in virulent macrophages compared to attenuated ones. The treatment with peptidimer-c decreases the expression level of Grb2 in virulent macrophages, whereas adding exogenous TGF-β2 to attenuated macrophages restores Grb2 expression. Right panel. Levels of Grb2 expression in Jed4 macrophages. (B) Western blot analysis with anti-Grb2 antibody using a whole cell lysates (10 μg of protein) derived from virulent and attenuated macrophages treated with TGF-β2 or not. Grb2 is diminished in attenuated macrophages and stimulation by TGF-β2 of attenuated macrophages restores protein levels compared to virulent Ode. Grb2 protein levels were normalized to β-actin protein levels. *p < 0.05 compared to virulent macrophages, #p < 0.05 compared to attenuated macrophages, §p < 0.05 compared to virulent macrophages treated with PEN.