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. 2015 Oct 29;5:15688. doi: 10.1038/srep15688

Figure 4. JAM-L is a downstream adhesion effector of Grb2/PI3K/AP-1 signaling.

Figure 4

Total RNA was extracted from virulent, engineered (delta169) virulent and attenuated Jed4 macrophages. Relative mRNA levels for jam-l were determined by qPCR and normalised to GAPDH transcripts. (A) Jam-l is transcriptionally up regulated in virulent compared to attenuated macrophages. Inhibition of Grb2 interactions and PI3-K signaling in virulent macrophages decreases the level of jam-l. (B) The level of jam-l transcription in engineered (delta169) Jed4 macrophages is reduced to that of attenuated Jed4 macrophages. (C) The protein expression levels of Fos-B and MMP9 in virulent macrophages is downregulated upon treatment with peptidimer-c. Fos-B and MMP9 protein levels were normalized to β-actin protein level. *p < 0.05 compared to virulent macrophages, §p < 0.05 compared to virulent macrophages treated with PEN.