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. 2015 Oct 29;5:15768. doi: 10.1038/srep15768

Figure 3. 5AZ increases the stability of IRF1 protein.

Figure 3

(A) Cells were stimulated with LPS for 4 hours followed by further treatment with MG132 and were harvested at the times shown. The immunoblots show the levels of IRF1 detected by using the IRF1 antibody; the data were analyzed by densitometry. As shown, upregulated IRF1 was rapidly decreased over time, whereas it remained highly expressed in the presence of MG132, a proteasome inhibitor. Results expressed as mean ± SD. *p < 0.05. (B) Cells were treated with LPS for 4 hours and additionally incubated at various time intervals to analyze IRF1 by immunoblot. After 4 hours, IRF1 protein was rapidly reduced, whereas the decrease was attenuated by 5AZ treatment. Results expressed as mean ± SD. *p < 0.05. (C) Cells were treated with LPS for 4 hours and further treated with LPS, MG132, or 5AZ as indicated for another 6 hours. IRF1 protein was dramatically decreased by LPS treatment for 10 hours, whereas MG132 almost completely blocked the degradation of IRF1. 5AZ treatment retarded the IRF1 degradation with statistical significance. Results expressed as mean ± SD. *Significantly different from cells treated with LPS for 10 hours, p < 0.05.