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. 2015 Oct 29;6:903. doi: 10.3389/fpls.2015.00903

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Copyright © 2015 Obrępalska-Stęplowska, Renaut, Planchon, Przybylska, Wieczorek, Barylski and Palukaitis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Real-time (quantitative) PCR (RT-qPCR) analysis of the changes in the levels of PSV RNAs and satRNA in Nicotiana benthamiana plants infected with biologically infectious transcripts of PSV-P plus satRNA (A), or PSV RNAs alone (B) at 21 dpi, comparing differences in accumulation between plants maintained at 27°C vs. 21°C. In (A), the change in 2a-encoding RNA was statistically insignificant and in the (B) the change in 1a-endoding RNA was statistically insignificant. The error bars represent standard errors.