Figure 5. Perfusion causes dramatic changes of protein phosphorylation in the mouse brain.

Mice were transcardially perfused with RT-PBS or cold PBS after sacrificed by cervical dislocation. The forebrains were collected and used for protein phosphorylation analysis by Western blots (A). The phosphorylation level was quantified after being normalized with the corresponding protein and presented as mean ± SEM (n = 4) (B). *^,#p < 0.05; **^,##p < 0.01; *perfusion vs control; ^#cold PBS vs RT-PBS.