Figure 6. Keeping tissues in ice-cold buffer prevents the alteration of protein phosphorylation in mouse brain.

Mice were sacrificed by dislocation. The fore brains were transferred immediately into ice-cold PBS (0 ) and kept in the ice-cold PBS for various time points. The protein phosphorylation was analyzed by Western blots (A). The phosphorylation levels of proteins were normalized by corresponding proteins and are presented as mean ± SEM (n = 4–5) (B). *p < 0.05.