Table 3.
Analysis of transposon integration sequences in human genomes induced by PGBD5
| Intact transposon | Mutant transposon | |||
|---|---|---|---|---|
| TTAA ITR | Non-ITR | TTAA ITR | Non-ITR | |
| Transposase | ||||
| GFP-PGBD5 | 82% (65)† | 18% (14) | 11% (4)‡ | 89% (33) |
| GFP Control | 17% (2) | 83% (10) | 40% (27) | 60% (40) |
Cells expressing GFP-PGBD5 and intact transposons exhibit significantly higher frequency of genomic integration as compared to either GFP control, or GFP-PGBD5 with mutant transposons, with 82% (65 out of 79) of sequences demonstrating DNA transposition of ITR transposons into TTAA sites (†p = 1.8 × 10-5). Mutation of the transposon ITR significantly reduces ITR-mediated integration, with only 11% (4 out of 37) of sequences (‡p = 0.0016). Numbers in parentheses denote absolute numbers of identified insertion sites.
GFP, green fluorescent protein; ITR, inverted terminal repeat.