Video 3. Spatio-temporal dynamics of de novo occurrence of a P− clone and its offspring.
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DOI: 10.7554/eLife.10811.009
Chromosomal DNA from strain P− green in which pilE is replaced by gfpmut3 is spread on the agar surface. Upon import and integration of DNA into a single bacterium, pili are lost and the bacterium becomes fluorescent. Time-lapse (Δt = 15 min) of de novo occurring P− green clone within an expanding colony. Brightfield image and fluorescence images were merged.