Table 1.
Bacterial strains and plasmids used in this study
| Strains | Description | Reference |
|---|---|---|
| E. coli DH5α | Plasmid-free E. coli | Amersham biosciences |
| C. glutamicum ATCC13032 | Wild-type strain | [60] |
| C. glutamicum ΔldhA | ATCC13032 with deletion of lactate dehydrogenase gene ldhA | This study |
| C. glutamicum ΔaceEΔpqoΔldhA | ATCC13032 with deletions of the genes for the E1 subunit of pyruvate dehydrogenase complex, the pyruvate:quinone oxidoreductase and the lactate dehydrogenase | [52] |
|
C. glutamicum ΔaceEΔpqo
ΔldhAΔmdh |
ATCC13032 with deletions of the genes for the E1 subunit of pyruvate dehydrogenase complex, the pyruvate:quinone oxidoreductase, lactate dehydrogenase and malate dehydrogenase | This study |
|
C. glutamicum ΔaceEΔpqo
ΔldhA (pEKEx2) |
C. glutamicum ΔaceEΔpqoΔldhA harboring plasmid pEKEx2 | This study |
| C. glutamicum ΔldhA (pEKEx2) | C. glutamicum ΔldhA harboring plasmid pEKEx2 | This study |
|
C. glutamicum ΔaceEΔpqo
ΔldhAΔmdh (pEKEx2) |
C. glutamicum ΔaceEΔpqoΔldhAΔmdh harboring plasmid pEKEx2 | This study |
| C. glutamicum ΔldhA (pEKEx2- als,aldB,butA) | C. glutamicum ΔldhA harboring plasmid pEKEx2 als,aldB,butA | This study |
|
C. glutamicum ΔaceEΔpqo
ΔldhA (pEKEx2-als,aldB,butA) |
C. glutamicum ΔaceEΔpqoΔldhA harboring plasmid pEKEx2-als,ald,BbutA | This study |
|
C. glutamicum ΔaceEΔpqo
ΔldhAΔmdh (pEKEx2-als,aldB,butA) |
C. glutamicum ΔaceEΔpqoΔldhAΔmdh harboring plasmid pEKEx2-als,aldB,butA | This study |
|
C. glutamicum ΔaceEΔpqo
ΔldhA (pEKEx2-als,aldB, Ptuf butA) |
C. glutamicum ΔaceEΔpqoΔldhA harboring plasmid pEKEx2-als,aldB, Ptuf butA | This study |
|
C. glutamicum ΔaceEΔpqo
ΔldhAΔmdh (pEKEx2-als,aldB, Ptuf butA) |
C. glutamicum ΔaceEΔpqoΔldhAΔmdh harboring plasmid pEKEx2-als,aldB, Ptuf butA | This study |
| Plasmids | ||
| pEKEx2 | kanR from pUC4 K; P trc , lacI, pUC18 mcs, induced by addition of IPTG | [61] |
| pEKEx2-als | pEKEx2 with cloned als from L. lactis | This study |
| pEKEx2-als,aldB,butA | pEKEx2-als with cloned aldB and butA from L. lactis | This study |
| pEKEx2-als,aldB, Ptuf butA | pEKEx2-als with cloned aldB and Ptuf-butA from L. lactis | This study |
als α-acetolactate synthase gene, aldB α-acetolactate decarboxylase gene, butA butanediol dehydrogenase gene, P tuf a 185 bp region upstream of tuf gene of C. glutamicum, kan R kanamycin resistance, P trc trc promoter, lacI lac repressor gene