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. 2015 Sep;10(9):1441–1449. doi: 10.4103/1673-5374.165512

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Protein and mRNA expression of Rho-kinase in the frontal cortex of chronic cerebral ischemia rats and the effects of Fasudil treatment.

(A) Protein expression of Rho-kinase 2 (ROCK2) was determined by western blot assay in the three [sham-operated (sham), ischemia, fasudil] groups at 3, 6, and 9 wk after surgery. (B) mRNA expression of ROCK2 was determined by RT-PCR in the three (sham, ischemia, fasudil) groups at 3, 6, and 9 wk after surgery. The mRNA and protein results are expressed as the ratio of the target gene or protein band intensity to that for β-actin. There were 10 rats at each time point per group. Data are presented as the mean ± SD and analyzed using one-way analysis of variance and the Student's t-test. *P < 0.05, vs. sham group; #P < 0.05, vs. ischemia group. wk: Weeks.