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Identificatiion of PCR products by enzymatic digestion.
M: DNA marker; 1: recombinant plasmid pIRES-BDNF (774 bp) by Xho I and Mlu I; 2: recombinant plasmid pIRES-CNTF (597 bp) by Xba I and Sal I; two fragments [i.e., target gene CNTF fragment (597 bp) and pIRES vector fragment (approximately 6.1 kb)] were acquired, confirming the product was recombinant plasmid pIRES-CNTF. BDNF: Brain-derived neurotrophic factor; CNTF: ciliary neurotrophic factor.
