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. 2015 Sep;10(9):1498–1506. doi: 10.4103/1673-5374.165523

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Identification of double-gene recon by PCR.

M: DNA marker; 1: double-gene recon 1 BDNF mRNA amplification product (788 bp); 2: double-gene recon 1 CNTF mRNA amplification product (611 bp); 3: double-gene recon 2 BDNF mRNA amplification product (774 bp); 4: double-gene recon 2 CNTF mRNA amplification product (597 bp). Recombinant plasmid pIRES-BDNF was digested by Xho I and Mlu I, and two fragments, a 774-bp-long target gene BDNF fragment and an approximately 1.6-kb-long pIRES vector fragment, were acquired, confirming the product was recombinant plasmid pIRES-BDNF. BDNF: Brain-derived neurotrophic factor. CNTF: ciliary neurotrophic factor.