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. 2015 Sep;10(9):1498–1506. doi: 10.4103/1673-5374.165523

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Identification of recombinant plasmid pIRES-BDNF-CNTF by enzymatic digestion.

1: DNA marker; 2: recombinant plasmid pIRES-BDNF-CNTF was digested by Xba I and Sal I, Xho I and Mlu I simutaneously; 3: recombinant plasmid pIRES-BDNF-CNTF was digested by Xho I and Mlu I; 4: recombinant plasmid pIRES-BDNF-CNTF was digested by Xba I and Sal I; 5: empty vector pIRES was digested by Xho I and Mlu I; 6: M: DNA marker. Enzymatic digestion products were visualized by gel electrophoresis using a 1.5% agarose gel. A 6.1-kb-sized vector band, a 774-bp-sized BDNF mRNA band and a 597-bp-sized CNTF band appeared, confirming the double-gene recombinant plasmid is pIRES-BDNF-CNTF. BDNF: Brain-derived neurotrophic factor; CNTF: ciliary neurotrophic factor.