Figure 4.

Effect of Tiansu and SMI silicone membranes on NSC differentiation (immunofluorescences staining, × 400).

(A, D, G) Control group. (B, E, H) Tiansu silicone membrane. (C, F, I) SMI silicone membrane. NSE⁺ cells had small bodies and long processes, GFAP⁺ cells had many long processes, while MBP⁺ cells were smaller in number, with small bodies, and short processes. In the control group, mature neurons and a large number of oligodendrocytes were visible. In the SMI silicone membrane group, there was a low rate of neuronal differentiation, with immature neurons, mature glial cells, and a small number of oligodendrocytes detected. The morphology in the Tiansu silicone membrane group was a combination of the control and SMI silicone membrane groups. NSC: Neural stem cell; NSE: neuron specific enolase; GFAP: glial fibrillary acidic protein; MBP: myelin basic protein; DAPI: 4′,6-diamidino-2-phenylindole.