Figure 9.

Down-regulating the expression of CELF1 could partially promote CDKN1B overexpression. A and B, Expression of CDKN1B protein was analysed by Western blot. C and D, Expression of CDKN1B mRNA was analysed by quantitative polymerase chain reaction. β-actin (AB) or GAPDH (CD) were used as loading controls. E, U87 and U251 cells were transiently transfected with CELF1-siRNA or CON-siRNA using Lipofectamine 2000. After 48 h, lysates were prepared and immunoprecipitated with anti-CUGBP1 or anti- CDKN1B antibody. Western blot was conducted with anti- CDKN1B or anti- CELF1 antibodies.