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. 2015 Oct 29;14:424. doi: 10.1186/s12936-015-0951-5

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© Dilshad et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Gel pictures showing results of normal and semiquantitative reverse transcriptase PCR: PCR amplified products of rol B (a), rol C (b) gene are shown in the figure. Semiquantitative RT-PCR showing the relative expression of rol B (c) and rol C gene (d). GADPH and β-actin used as housekeeping gene (e, f). TB1–TB3 represents rol b transgenics whereas TC1–TC3 represent rol C transgenics. WT stands for wild type plant of A. annua, lane P refers to the plasmid DNA and lane M corresponds to the marker DNA (Fermentas). NT indicates the negative or “no RT control” for each transgenic line