Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 21;39(12):1733–1741. doi: 10.1038/ijo.2015.121

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

PMC Copyright notice

Effects of MEST depletion on phosphorylation of CREB/ATF1 and intracellular cAMP and cGMP levels. hMADS cells were transfected at confluence (d-2) with siRNAs targeting MEST (siMEST) or a control siRNA (siC). Adipocyte differentiation was induced 2 days later (d0) using complete differentiation medium. (a) Protein lysates of cells at d1, d2 and d3 were analysed by western blotting for phosphorylation of CREB and ATF1 (p-CREB and p-ATF-1), total CREB, MEST and β-Tubulin (Tubβ). Numbers above p-CREB bands reflect the results of densitometric analysis (ratio of p-CREB/CREB) and are presented relative to siC-transfected cells at d1. (b and c) Cells at d0 and d2 were analysed for intracellular levels of cAMP (b) and cGMP (c). Testing for significant differences in second messenger levels was performed by a paired t-test; *P<0.05.