Figure 4.

In vivo GCaMP2 fluorescence changes in the renal proximal tubular cells show the elevation of calcium level after intraarterial addition of ATP and AngII—these effects are blocked by the respective receptor inhibitors. (A and B) GCaMP2 fluorescence changes after the intraarterial addition of ATP 3.25 mg/kg (100 µM)—effect of 5-minute pretreatment with suramin 25 mg/kg (300 µM). (C and D) GCaMP2 fluorescence changes after the intraarterial addition of AngII 50 µg/kg (0.75 µM)—effect of 5-minute pretreatment with 28 µg/kg candesartan (1 µM).